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Arthur E. Johnson

Arthur E.Johnson


Regents Professor

Distinguished Professor of Chemistry
Distinguished Professor of Molecular and Cellular Medicine
Professor, Department of Biochemistry and Biophysics

College of Medicine
142E Reynolds Medical Building
Texas A&M Health Science Center
1114 TAMU
College Station, Texas 77843-1114
Phone: 979-862-3440
Fax: 979-847-9481

Email:ajohnson@medicine.tamhsc.edu

Education

  • University of Oregon, Eugene, Oregon, 9/69 to 11/73. Ph.D. in Chemistry, with a major in Biochemistry/Molecular Biology. Mentor: John R. Menninger.
  • California Institute of Technology, Pasadena, California, 9/60 to 6/64. B.S. in Chemistry. Undergraduate research in the laboratory of Norman Davidson.

Experience

Texas A&M Health Science Center, College Station, TX

  • Regents Professor, Texas A&M Health Science Center, 12/08 to present
  • Distinguished Professor of Molecular and Cellular Medicine, 3/04 to present
  • E.L. Wehner-Welch Foundation Chair in Chemistry, 9/94 to 2012
  • Professor of Medical Biochemistry & Genetics, 9/94 to 3/04
  • Director, Molecular Biophysics Training Program, 2/00 to 2/05; member, 2/05 to 6/08
  • Mechanistic Studies at the Host-Pathogen Interface Training Program, 7/03 to present

Texas A&M University, College Station, TX

  • Distinguished Professor of Chemistry, 9/02 to present
  • Professor of Biochemistry and Biophysics, 9/95 to present
  • Professor of Chemistry, 9/94 to 8/02
  • Chemistry-Biology Interface Training Program, 9/95 to 5/02
  • Center for Advanced Biomolecular Research, 4/00 to 5/06

(more experience...)

Honors

  • Fritz Lipmann Lectureship, American Society of Biochemistry and Molecular Biology, 2011
  • University of Oregon Alumni Achievement Award in Chemistry, 2009
  • Appointed Regents Professor in the Texas Texas A&M Health Science Center, 2008
  • JoAnn Treat Research Excellence Award, Texas A&M Research Foundation, 2006
  • Elected a Fellow of AAAS, the American Association for the Advancement of Science, 2004
  • Distinguished Professor, Texas A&M Health Science Center, 2004
  • Distinguished Professor, Texas A&M University, 2002
  • Senior Investigator Excellence in Research Award, College of Medicine, Texas A&M Health Science Center, 2000
  • Sigma Xi Distinguished Scientist, Texas A&M University, 2000

(more honors...)

Full-length Research Papers and Reviews 

  • Malhotra, K., Sathappa, M., Landin, J. S., Johnson, A. E., and Alder, N. N. (2013) "Structural Changes in the Mitochondrial Tim23 Channel Are Coupled to the Protonmotive Force," Nature Structural and Molecular Biology, in press.
  • Sato, T. K., Tweten, R. K., and Johnson, A. E.* (2013) "Disulfide-Bond Scanning Reveals Assembly State and β--Strand Tilt Angle of PFO β--Barrel," Nature Chemical Biology, 9, 383-389.
  • Hou, B., Lin, P.-J., and Johnson, A. E. (2012) "Membrane Protein TM Segments Are Retained at the Translocon during Integration Until the Nascent Chain Cues FRET-Detected Release into the Lipid Phase," Molecular Cell 48, 398-408.
  • Hotze, E. M., Wilson-Kubalek, E., Farrand, A. J., Bentsen, L., Parker, M. W., Johnson, A. E., and Tweten, R. K. (2012) "Monomer-Monomer Interactions Propagate Structural Transitions Necessary for Pore Formation by the Cholesterol-Dependent Cytolysins," Journal of Biological Chemistry 287, 24534-24543.
  • Wu, C., Wei, J., Lin, P.-J., Tu, L., Deutsch, C., Johnson, A. E., and Sachs, M. S. (2012) “Arginine Changes the Conformation of the Arginine Attenuator Peptide Relative to the Ribosome Tunnel,”Journal of Molecular Biology 416, 518-533.
  • Lin, P.-J., Jongsma, C . G., Pool, M. R., and Johnson, A. E. (2011) "Polytopic Membrane Protein Folding at L17 in the Ribosome Tunnel Initiates Cyclical Changes at the Translocon,"  Journal of Cell Biology 195, 55-70.
  • Lin, P.-J., Jongsma, C. G., Liao, S., and Johnson, A. E. (2011) "Transmembrane Segments of Nascent Polytopic Membrane Proteins Control Cytosol/ER Targeting during Membrane Integration ," Journal of Cell Biology 195, 41-54.
  • Devaraneni, P., Conti, B., Matsumura, Y., Yang, Z., Johnson, A. E., and Skach, W. R. (2011) "Stepwise Insertion and Inversion of a Type II Signal Anchor Occurs in the Ribosome-Sec61 Complex" Cell 146, 134-147.
  • Cain, P., Holdermann, I., Sinning, I., Johnson, A. E., and Robinson, C. (2011) “Binding of Chloroplast Signal Recognition Particle to a Thylakoid Membrane Protein Substrate in Aqueous Solution and Delineation of the cpSRP43-Substrate Interaction Domain,” Biochemical Journal, 437, 149-155.
  • Khushoo, A., Yang, Z., Johnson, A. E., and Skach, W. R. (2011) "Ligand-Driven Vectorial Folding of Ribosome-Bound Human CFTR NBD1," Molecular Cell 41, 682-692.
  • Tamborero, S., Vilar, M., Martínez-Gil, L., Johnson, A. E., and Mingarro, I. (2011) "Membrane Insertion and Topology of the TRanslocating Chain-Associating Membrane (TRAM) Protein," Journal of Molecular Biology 406, 571-582.
  • Gubbens, J., Kim, S. J., Yang, Z., Johnson, A. E., and Skach, W. R. (2010) "Efficient In Vitro Incorporation of Non-natural Amino Acids into Protein Using tRNACys-derived Opal, Ochre, and Amber Suppressor tRNAs," RNA 16, 1660-1672.
  • Zhang, Z., Zhu, W. Lapolla, S. M., Miao, Y., Shao, Y., Falcone, M., Johnson, A. E., Zhang, X. C., Andrews, D. W., and Lin, J. (2010) "Bax Forms an Oligomer by a Sequential Mechanism Utilizing Separate, Yet Interdependent, Surfaces," Journal of Biological Chemistry 285, 17614-17627.
  • Martínez-Gil, L., Johnson, A. E., and Mingarro, I. (2010) “Membrane Insertion and Biogenesis of the Turnip Crinkle Virus p9 Movement Protein,” Journal of Virology 84, 5520-5527.
  • Mary, C., Scherrer, A., Huck, L. Lakkaraju, A. K. K., Thomas, Y., Johnson, A. E., and Strub, K. (2010) "Residues in SRP9/14 Essential for Elongation Arrest Activity of the Signal Recognition Particle Define a Positively Charged Functional Domain on One Side of the Protein," RNA 16, 969-979.
  • Farrand, A. J., LaChapelle, S., Hotze, E., Johnson, A. E., and Tweten, R. K. (2010) "Only Two Amino Acids Are Essential for Cytolytic Toxin Recognition of Cholesterol at the Membrane Surface," Proceedings of the National Academy of Sciences (USA) 107, 4341-4346.
  • Johnson, A. E. (2009) “The Structural and Functional Coupling of Two Molecular Machines, the Ribosome and the Translocon,” Journal of Cell Biology 185, 765-767.
  • Flanagan, J. J., Tweten, R. K., Johnson, A. E., and Heuck, A. P. (2009) “Cholesterol Exposure at the Membrane Surface Is Necessary and Sufficient to Trigger Perfringolysin O Binding,” Biochemistry 48, 3977-3987.
  • Mayerhofer, P. U., Cook, J. P., Wahlman, J., Pinheiro, T. T. J., Moore, K. A. H., Lord, J. M., Johnson, A. E., and Roberts, L. M. (2009) “Ricin A-Chain Insertion into ER Membranes Is Triggered by a Temperature Increase to 37°C,” Journal of Biological Chemistry 284, 10232-10242.
  • Saurí, A., Tamborero, S., Martínez-Gil, L., Johnson, A. E., and Mingarro, I. (2009) “Viral Membrane Protein Topology Is Dictated by Multiple Determinants in Its Sequence,” Journal of Molecular Biology, in press.
  • Berndt, U., Oellerer, S., Zhang, Y., Johnson, A. E., and Rospert, S. (2009) “A Signal-Anchor Sequence Stimulates Signal Recognition Particle Binding to Ribosomes from Inside the Exit Tunnel,” Proceedings of the National Academy of Sciences (USA) 106, 1398-1403.
  • Pitonzo, D., Yang, Z., Rusterholtz, K., Johnson, A. E., and Skach, W. R. (2009) “Sequence-Specific Retention and Regulated Release of a Nascent Translocating Polypepetide by the ER Sec61 Translocon,” Molecular Biology of the Cell 20, 685-698.
  • Saksena, S., Wahlman, J., Teis, D., Johnson, A. E., and Emr, S. D. (2009) “Functional Reconstitution of ESCRT-III Assembly and Disassembly,” Cell 136, 97-109.
  • Daniel, C., Conti, B., Johnson, A. E., and Skach, W. R. (2008) “Control of Translocation through the Sec61 Translocon by Nascent Polypeptide Structure within the Ribosome,” Journal of Biological Chemistry 283, 20864-20873.
  • Alder, N. N., Jensen, R. E., and Johnson, A. E. (2008) “Fluorescence Mapping of Mitochondrial TIM23 Complex Reveals a Water-Facing, Substrate-Interacting Helix Surface,” Cell 134, 439-450.
  • Lakkaraju, A. K. K., Mary, C., Scherrer, A., Johnson, A. E., and Strub, K. (2008) “SRP Maintains Nascent Chains Translocation-Competent by Slowing Translation Rates to Match Limiting Numbers of Targeting Sites,” Cell 133, 440-451.
  • Nelson, L., Johnson, A. E., and London, E. (2008) “How the Interaction of Perfringolysin O with Membranes Is Controlled by Sterol Structure, Lipid Structure, and Physiological Low pH: Insights into the Origin of Perfringolysin O-Lipid Raft Interaction,” Journal of Biological Chemistry 283, 4632-4642.
  • Alder, N. N., Sutherland, J., Buhring, A. I., Jensen, R. E., and Johnson, A. E. (2008) “Quaternary Structure of the Mitochondrial TIM23 Complex Reveals Dynamic Association between Tim23p and Other Subunits,” Molecular Biology of the Cell 19,159-170.
  • Bowden, M. G., Heuck, A. P., Ponnuraj, K., Kolosova, E., Choe, D., Gurusiddappa, S., Narayana, S. V. L., Johnson, A. E., and Höök, M. (2008) “Evidence for the “Dock, Lock, and Latch” Ligand Binding Mechanism of the Staphylococcal MSCRAMM SDRG,” Journal of Biological Chemistry 283, 638-647.
  • Soltani, C. E., Hotze, E. M., Johnson, A. E., and Tweten, R. K. (2007) “Structural Elements of the Cholesterol-Dependent Cytolysins that Are Responsible for their Cholesterol-Sensitive Membrane Interactions,” Proceedings of National Academy of Science (USA) 104, 20226-20231.
  • Davis, A. J., Alder, N. N., Jensen, R. E., and Johnson, A. E. (2007) “The Tim9p/10p and Tim8p/13p Complexes Bind to Specific Sites on Tim23p during Mitochondrial Protein Import,” Molecular Biology of the Cell 18, 475-486.
  • Saurí, A., McCormick, P. J., Johnson, A. E., and Mingarro, I. (2007) “Sec61α and TRAM Are Sequentially Adjacent to a Nascent Viral Membrane Protein during Its Integration,” Journal of Molecular Biology 366, 366-374.
  • Lakshmipathy, S. K., Tomic, S, Kaiser, C. M., Chang, H.-C., Genevaux, P., Georgopoulous, C., Barral, J. M., Johnson, A. E., Hartl, F. U., and Etchells, S. A. (2007) “Identification of Nascent Chain Interaction Sites on Trigger Factor,” Journal of Biological Chemistry 282, 12186-12193.
  • Wahlman, J., DeMartino, G. N., Skach, W. R., Bulleid, N. J., Brodsky, J. L., and Johnson, A. E. (2007) “Real-time Fluorescence Detection of ERAD Substrate Retro-translocation in a Mammalian In Vitro System,” Cell 129, 943-955
  • Soltani, C. E., Hotze, E. M., Johnson, A. E., and Tweten, R. K. (2007) “Specific Protein-Membrane Contacts Are Required for Prepore and Pore Assembly by a Cholesterol-Dependent Cytolysin,” Journal of Biological Chemistry 282, 15709-15216
  • Heuck, A. P., Savvo, C. G., Holzenburg, A., and Johnson, A. E. (2007) “Conformational Changes that Effect Oligomerization and Initiate Pore Formation Are Triggered throughout Perfringolysin O upon Binding to Cholesterol,” Journal of Biological Chemistry 282, 22629-22637.
  • Lundin, C., Johansson, S., Johnson, Arthur E., Näslund, J., von Heijne, G., Nilsson, IM. (2007) “Stable insertion of alzheimer Aβ peptide into the ER membrane strongly correlates with its length,” FEBS Letters 581, 3809-3813.
  • Tomic, S., Johnson, A. E., Hartl, F. U., and Etchells, S. A. (2006) "Exploring the Capacity of Trigger Factor to Function as a Shield for Ribosome-Bound Polypeptide Chains," FEBS Letters 580, 72-76.
  • Seo, J., Barhoumi, R., Johnson, A. E., Lupton, J. R., and Chapkin, R. S. (2006) "Docosahexaenoic Acid Selectively Inhibits Plasma Membrane Targeting of Lipidated Proteins," FASEB Journal 20, 770-772.
  • Frankel, D. J., Pfeiffer, J. R., Surviladze, Z., Johnson, A. E., Oliver, J. M., Wilson, B. S., and Burns, A. R. (2006) "Revealing the Topography of Membrane Domains by Combined AFM/Fluorescence Imaging," Biophysical Journal 90, 2404-2413.
  • Araç, D., Chen, X., Khant, H. A., Ubach,J., Ludtke, S. J., Kikkawa, M., Johnson, A. E., Chiu, W., Südhof, T. C., and Rizo, J. (2006) "Membrane-Membrane Proximity Induced by Ca2+-Dependent Multivalent Binding of Synaptotagmin 1 to Phospholipids," Nature Structural & Molecular Biology 13, 209-217.
  • Saksena, S., Summers, M. D., Burks, J. K., Johnson, A. E., and Braunagel, S. C. (2006) “Importin α-16: a Translocon-Associated Protein that May Facilitate Sorting of Integral Membrane Proteins to the Nuclear Envelope,” Nature Structural & Molecular Biology 13, 500-508.
  • Woolhead, C. A., Johnson, A. E., and Bernstein, H. D. (2006) “Translation Arrest Requires Two-Way Communication between a Nascent Polypeptide and the Ribosome,” Molecular Cell 22, 587-598.
  • Johnson, A. E. (2005) "The Co-Translational Folding and Interactions of Nascent Protein Chains: A New Approach Using Fluorescence Resonance Energy Transfer," FEBS Letters 579, 916-920. Nobel Symposium special issue.
  • Alder, N.N., Shen, Y., Brodsky, J.L., Hendershot, L.M., and Johnson, A.E. (2005) "The Molecular Mechanisms Underlying BiP-Mediated Gating of the Sec61 Translocon of the Endoplasmic Reticulum," Journal of Biological Chemistry 168, 389-399.
  • Heuck, A.P. and Johnson, A.E. (2005) "Membrane Recognition and Pore Formation by Bacterial Pore-Forming Toxins" in Protein-Lipid Interactions (L.K. Tamm, editor), Wiley-VHC Weinheim, 163-186.
  • Giddings, K.S., Johnson, A.E., and Tweten, R.K. (2005) "Perfringolysin O and Intermedilysin: Mechanisms of Pore Formation by the Cholesterol-Dependent Cytolysins," in The Comprehensive Sourcebook of Bacterial Protein Toxins, Third Edition (J.E. Alouf and M.R. Popoff, editors), Academic Press, Oxford, England, in press.
  • Ramachandran, R., Tweten, R.K., and Johnson, A.E. (2005) "The Domains of a Cholesterol-Dependent Cytolysin Undergo a Major FRET-Detected Rearrangement during Pore Formation," Proceedings of the National Academy of Sciences (USA) 102, 7139-7144.
  • Saurí, A., Saksena, S., Salgado, J., Johnson, A. E., and Mingarro, I. (2005) "Double-Spanning Plant Viral Movement Protein Integration into the ER Membrane Is SRP-Dependent, Translocon-Mediated, and Concerted," Journal of Biological Chemistry 280, 25907-25912.
  • Etchells, S.A., Meyer, A.S., Yam, A.Y., Roobol, A., Miao, Y., Shao, Y., Carden, M.J., Skach, W.R., Frydman, J., and Johnson, A.E. (2005) "The Cotranslational Contacts between Ribosome-Bound Nascent Polypeptides and the Subunits of the Hetero-Oligomeric Chaperonin TRiC Probed by Photocrosslinking," Journal of Biological Chemistry 280, 28118-28126.
  • Johnson, A.E. (2005) "Fluorescence Approaches for Determining Protein Conformations, Interactions and Mechanisms at Membranes," Traffic 6, 1078-1982.
  • Karamyshev, A.L., and Johnson, A.E. (2005) "Selective SecA Association with Signal Sequences in Ribosome-Bound Nascent Chains: A Potential Role for SecA in Ribosome Targeting to the Bacterial Membrane," Journal of Biological Chemistry 280, 37930-37940.
  • Sadlish, H., Pitonzo, D., Johnson, A.E., and Skach, W.R. (2005) "Sequential Triage of Transmembrane Segments by Sec61α During Biogenesis of a Native Multispanning Membrane Protein," Nature Structural & Molecular Biology 12, 870-878.
  • Karamyshev, A. L., Kelleher, D. J., Gilmore, R., Johnson, A. E., von Heijne, G., and Nilsson, I. (2005) "Mapping the Interaction of the STT3 Subunit of the Oligosaccharyltransferase Complex with Nascent Polypeptide Chains," Journal of Biological Chemistry 280, 40489-40493.
  • Johnson, A.E. and Jensen, R.E. (2004) "Barreling through the Membrane," Nature Structural & Molecular Biology 11, 113-114.
  • Woolhead, C., McCormick, P.J., and Johnson, A.E. (2004) "Nascent Membrane and Secretory Proteins Differ in FRET-Detected Folding Far Inside the Ribosome and in their Exposure to Ribosomal Proteins," Cell 116, 725-736.
  • Alder, N.N. and Johnson, A.E. (2004) "Cotranslational Membrane Protein Biogenesis at the Endoplasmic Reticulum," Journal of Biological Chemistry 279, 22787-22790.
  • Ramachandran, R., Tweten, R. K., and Johnson, A. E. (2004) “Membrane-Dependent Conformational Changes Initiate Cholesterol-Dependent Cytolysin Oligomerization and Intersubunit β-Strand Alignment,” Nature Structural & Molecular Biology 11, 697-705.
  • Johnson, A. E. (2004) “Functional Ramifications of FRET–Detected Nascent Chain Folding Far Inside the Membrane–Bound Ribosome,” Biochemical Society Transactions 32, 668-672.
  • Saksena, S., Shao, Y., Braunagel, S., Summers, M. D., and Johnson, A. E. (2004) "Cotranslational Integration and Initial Sorting at the ER Translocon of Proteins Destined for the Inner Nuclear Membrane," Proceedings of the National Academy of Sciences (USA) 101, 12537-12542.
  • Zhang, Z., Lapolla, S. M., Annis, M. G., Truscott, M., Roberts, G. J., Miao, Y., Shao, Y., Tan, C., Peng, J., Johnson, A. E., Zhang, X. C., Andrews, D. W., and Lin, J. (2004) “Bcl-2 Homodimerization Involves Two Distinct Binding Surfaces, a Topographic Arrangement that Provides an Effective Mechanism for Bcl-2 to Capture Activated Bax,” Journal of Biological Chemistry 279, 43920-43928.
  • Huck, L., Scherrer, A., Terzi, L., Johnson, A. E., Bernstein, H. D., Cusack, S., Weichenrieder, O., and Strub, K. (2004) “Conserved Tertiary Base Pairing Ensures Proper RNA Folding and Efficient Assembly of the Signal Recognition Particle Alu Domain,” Nucleic Acids Research 32, 4915-4924.
  • Ramachandran, R., Heuck, A.P., Tweten, R.K., and Johnson, A.E. (2002) "Structural Insights into the Membrane-Anchoring Mechanism of a Cholesterol-Dependent Cytolysin," Nature Structural Biology 9, 823-827.
  • Daniels, R., Kurowski, B., Johnson, A.E., and Hebert, D.N. (2003) "N-linked Glycans Direct the Co-Translational Folding Pathway of Influenza Hemagglutinin," Molecular Cell 11, 79-90.
  • Mori, H., Tsukazaki, T., Masui, R., Kuramitsu, S., Yokoyama, S., Johnson, A.E., Kimura, Y., Akiyama, Y., and Ito, K. (2003) "Fluorescence Resonance Energy Transfer Analysis of Protein Translocase: SecYE from Thermus thermophilus HB8 Forms a Constitutive Oligomer in Membranes," Journal of Biological Chemistry 278, 14257-14264
  • Flanagan, J.J., Chen, J.-C., Miao, Y., Shao, Y., Lin, J., Bock, P.E., and Johnson, A.E. (2003) "SRP Binds to Ribosome-bound Signal Sequences with Fluorescence-detected Subnanomolar Affiity that Does Not Diminish as the Nascent Chain Lengthens," Journal of Biological Chemistry 278, 18628-18637.
  • Nilsson, I., Kelleher, D.J., Miao, Y., Shao, Y., Kreibich, G., Gilmore, R., von Heijne, G., and Johnson, A.E. (2003) "Photocrosslinking of Nascent Chains to the STT3 Subunit of the Oligosaccharyltransferase Complex," Journal of Cell Biology 161, 715-725.
  • Leach, J.K., O'Rear, E.A., Patterson, E., Miao, Y., and Johnson, A.E. (2003) "Accelerated Thrombolysis in a Rabbit Model of Carotid Artery Thrombosis with Liposome-Encapsulated and Microencapsulated Streptokinase," Thrombosis and Haemostasis 90, 64-70.
  • Nilsson, I., Johnson, A.E., and von Heijne, G. (2003) "How Hydrophobic is Alanine?" Journal of Biological Chemistry 278, 29389-29393.
  • Heuck, A.P., Tweten, R.K., and Johnson, A.E. (2003) "Assembly and Topography of the Prepore Complex in Cholesterol-Dependent Cytolysins," Journal of Biological Chemistry 278, 31218-31225.
  • Johnson, A.E. (2003) "Maintaining the Permeability Barrier during Protein Trafficking at the ER Membrane," Biochemical Society Transactions 31, 1227-1231.
  • McCormick, P.J., Miao, Y., Shao, Y., Lin, J., and Johnson, A.E. (2003) "Cotranslational Protein Integration into the ER Membrane Is Mediated by the Binding of Nascent Chains to Translocon Proteins," Molecular Cell 12, 329-341.
  • Giddings, K. S., Johnson, A. E., and Tweten, R. K. (2003) "Redefining Cholesterol's Role in the Mechanism of the Cholesterol-Dependent Cytolysins," Proceedings of the National Academy of Sciences (USA) 100, 11315-11320.
  • Heuck, A.P., and Johnson, A.E. (2002) "Pore-Forming Protein Structure Analysis in Membranes Using Multiple Independent Fluorescence Techniques," Cell Biochemistry and Biophysics 36, 89-101.
  • Haigh, N. G., and Johnson, A. E. (2002) "A New Role for BiP: Closing the Aqueous Translocon Pore during Protein Integration into the ER Membrane," Journal of Cell Biology 156, 261-270.
  • Haigh, N.G., and Johnson, A.E. (2002) "Protein Sorting at the Membrane of the Endoplasmic Reticulum," in Protein Targeting, Transport, and Translocation (Dalbey, R., and von Heijne, G., eds.), pp. 74-106, Academic Press, London.
  • Hotze, E.M., Heuck, A.P., Czajkowsky, D., Shao, Z., Johnson, A.E., and Tweten, R.K. (2002) "Monomer-Monomer Interactions Drive the Prepore to Pore Conversion of a β-Barrel-Forming Cholesterol-Dependent Cytolysin," Journal of Biological Chemistry 277, 11597-11605.
  • Nilsson, I., Johnson, A.E., and von Heijne, G. (2002) "Cleavage of a Tail-Anchored Protein by Signal Peptidase," FEBS Letters, 516, 106-108.
  • Pariyarath, R., Wang, H., Aitchison, J. D., Ginsberg, H. N., Johnson, A. E., and Fisher, E. A. (2001) "Co-translational Interactions of Apoprotein B with the Ribosome and Translocon during its Assembly with Lipids in the Endoplasmic Reticulum or its Targeting to Proteasomal Degradation in the Cytosol," Journal of Biological Chemistry 276, 541-550.
  • Tweten, R. K., Parker, M. W., and Johnson, A. E. (2001) "The Cholesterol-Dependent Cytolysins," in Pore-Forming Toxins (van der Goot, G., ed.), pp. 15-33, Springer-Verlag, Heidelberg.
  • Hotze, E. M., Kubalek, E., Rossjohn, J., Parker, M., Johnson, A. E., and Tweten, R. K. (2001) "Arresting Pore Formation of a Cholesterol-Dependent Cytolysin by Disulfide Trapping Synchronizes the Insertion of the Transmembrane β-Sheet from a Prepore Intermediate," Journal of Biological Chemistry 276, 8261-8268
  • Vidugiriene, J., Vainauskas, S., Johnson, A. E., and Menon, A. K. (2001) "Endoplasmic Reticulum Proteins Involved in Glycosylphosphatidylinositol Anchor Attachment: Photocrosslinking Studies in a Cell-Free System," European Journal of Biochemistry 268, 2290-2300.
  • Heuck, A.P., Tweten, R.K., and Johnson, A.E. (2001) "β-Barrel Pore-Forming Toxins: Intriguing Dimorphic Proteins," Biochemistry 40, 9065-9073.
  • Nilsson, I., Ohvo-Rekilä, H., Slotte, J.P., Johnson, A.E., and von Heijne, G. (2001) "Inhibition of Protein Translocation across the ER Membrane by Sterols," Journal of Biological Chemistry 276, 41748-41754.
  • Jensen, R.E., and Johnson, A.E. (2001) "Opening the Door to Mitochondrial Protein Import," Nature Structural Biology 8, 1008-1010.
  • Johnson, A.E., Chen, J.-C., Flanagan, J.J., Miao, Y., Shao, Y., Lin, J., and Bock, P.E. (2001) "Structure, Function, and Regulation of Free and Membrane-bound Ribosomes: The View from their Substrates and Products," in The Ribosome, Cold Spring Harbor Symposia on Quantitative Biology 66, 531-541.
  • Lain, B., Tanase, C. A., Iriarte, A., Johnson, A. E., and Martinez-Carrion, M. (2000) "Association of Newly Synthesized Mitochondrial Aspartate Aminotransferase with Cytosolic Factors," in Vitamin B6-Dependent Proteins and PQQ and Quinoproteins (Martinez-Carrion, M., Iriarte, A., and Kagan, H., eds.), pp. 245-249, Birhauser Verlag, Basel, Switzerland.
  • McCallum, C.D., Do, H., Johnson, A.E., and Frydman, J. (2000) "The Interaction of the Chaperonin Tailless Complex Polypeptide 1 (TCP1) Ring Complex (TRiC) with Ribosome-bound Nascent Chains Examined Using Photo-cross-linking," Journal of Cell Biology 149, 591-601.
  • Peluso, P., Herschlag, D., Freymann, D.M., Johnson, A.E., Walter, P. (2000) "Role of 4.5S RNA in Assembly of the Bacterial Signal Recognition Particle with Its Receptor," Science 288, 1640-1643.
  • Hackeng, T.M., Yegneswaran, S., Johnson, A.E., Griffin, J.H. (2000) "Conformational Changes in Activated Protein C Caused by Binding of the First Epidermal Growth Factor-Like Module of Protein S," Biochemical Journal 349, 757-764.
  • Shepard, L.A., Shatursky, O., Johnson, A.E., and Tweten, R.K. (2000) "The Mechanism of Pore Assembly for a Cholesterol-Dependent Cytolysin: Formation of a Large Prepore Complex Precedes the Insertion of the Transmembrane β-Hairpins," Biochemistry 39, 10284-10293.
  • Davis, A.J., Holder, J., Sepuri, N.B., Johnson, A.E., and Jensen, R.E. (2000) "Two Intermembrane Space TIM Complexes Interact with Different Domains of Tim23p durings its Import Into Mitochondria," Journal of Cell Biology 150, 1271-1281.
  • Johnson, A.E. and Haigh, N.G. (2000) "The ER Translocon and Retro-Translocation: Is the Shift into Reverse Manual or Automatic?" Cell 102, 709-712.
  • Heuck, A.P., Hotze, E.M., Tweten, R.K., and Johnson, A.E. (2000) "Mechanism of Membrane Insertion of Mutlimeric β-Barrel Protein: Perfringolysin O Creates a Pore Using Ordered and Coupled Conformational Changes," Molecular Cell 6, 1233-1242.

(more publications...)

U.S. and International Patents

  • Skach, W. R., Yang, Z., Kim, S.-J., Gubbens, J., and Johnson, A. E. “Use of Cysteine-derived Suppressor tRNAs for Non-Native Amino Acid Incorporation.” U. S. Patent pending.
  • O'Rear, E. A., Nguyen, P. D., Johnson, A. E., Patterson, E., and Whitsett, T. L., "Method and Composition for the Treatment of Thrombosis in a Mammal."
  • U.S. Patent #5,503,850 Issued 4/2/96
  • European Patent #0472657
  • Canadian Patent #2,016,949 Issued 12/10/96

Ph.D. Dissertations Directed by Dr. Johnson

University of Oklahoma, Department of Chemistry and Biochemistry

  • Adkins, Harvey J., 1982. "An Investigation of the Interaction of Aminoacyl-Transfer RNA with Elongation Factor Tu Using Site-Specific Fluorescent Probes."
  • Abrahamson, Julie K., 1984. "An Investigation of Protein-Nucleic Acid Interactions: The Affinity of Aminoacyl-Transfer RNA for Elongation Factor Tu."
  • Isaacs, Benjamin S., 1985. "Structural Aspects of Blood Coagulation Proteins and Their Complexes: A Fluorescence Spectroscopic Study."
  • Dell, Virginia Ann, 1987. "Elongation Factor Conformation: Its Effect on the Aminoacyl-tRNAlElongation FactorlGTP Ternary Complex as Detected Using a Fluorescent-Labeled Transfer RNA."
  • Husten, E. Jean, 1988. "The Molecular Architecture of Membrane-Bound Enzyme-Cofactor Complexes Involved in Blood Coagulation."
  • Krieg, Ute C., 1989. "Photocrosslinking Studies of the Environment of Nascent Polypeptides During Protein Synthesis and Secretion."
  • Janiak, Fabiola, 1991. "Assembly of the Signal Recognition Particle: A Study of Its Protein-Nucleic Acid and Protein-Protein Interactions Using Fluorescence Spectroscopy."
  • Shore, Steven K., 1991."The Use of Fluorescent-labeled Amino Acids to Examine the Environment of Ribosome-bound Nascent Polypeptide Chains."
  • Ye, Jia, 1992. "Structure-Function Relationships of the Anticoagulant Thrombin-Thrombomodulin Complex."
  • Crowley, Kathleen S., 1993. "The Secretory Protein Pathway within the Ribosome and through the Membrane of the Endoplasmic Reticulum."
  • Mutucumarana, Vasantha P., 1993. "The Active Site of Membrane-bound Blood Coagulation Factor IXa: Its Location above the Membrane Surface and its Conformational Sensitivity to the Factor VIIIa Cofactor."
  • Dharmawardana, Kumudini, 1994. "The Sensitivity of the Active Site Conformation and Topography of Membrane-bound Factor Xa to Individual Factor Va Subunits and to Cofactor Inactivation."

Texas A&M University and Texas A&M Health Science Center

  • Do, Hung V., 1997. "Photocrosslinking Studies of the Co-translational Integration of Nascent Membrane Proteins into the Endoplasmic Reticulum Membrane." Ph. D. in Medical Sciences.
  • Liao, Shuren, 1997. "A Fluorescence Examination of Nascent Polypeptide Environment and the Ribosome-Membrane Junction during Co-translational Protein Translocation and Integration at the ER Membrane." Ph. D. in Medical Sciences.
  • Yegneswaran, Subramanian, 1997. "The Molecular Architecture of the Anticoagulant Membrane-Bound Activated Protein C Enzyme and Its Dependence on the Cofactor Protein S and Phospholipid Composition." Ph. D. in Medical Sciences.
  • Chen, Jui-Chang, 1997. "Signal Recognition Particle Interactions with the Signal Sequence, Nascent Chains, Ribosomes, and GTP/GDP." Ph. D. in Medical Sciences.
  • Flanagan, John, 2003. "Detecting Macromolecular Associations By Fluorescence Spectroscopy." Ph.D. in Biochemistry and Biophysics.
  • Etchells, Stephanie, 2003. "Examination of Eukaryotic Chaperonin-Mediated Nascent Chain Folding in the Cytosol: A Photocrosslinking Approach." Ph.D. in Biochemistry and Biophysics.
  • McCormick, Peter, 2003. "Investigating Cotranslational Protein Integration into the Endoplasmic Reticulum Membrane." Ph.D. in Biochemistry and Biophysics.
  • Ramachandran, Rajesh, 2004. "Structural Insights into the Mechanisms of Membrane Binding and Oligomerization of a Bacterial Pore-Forming Toxin." Ph.D. in Biochemistry and Biophysics.
  • Wahlman, Judit 2007.  "Fluorescent-detected Retrotranslocation of an Endoplasmic Reticulum-Associated (ERAD) Substrate in a Mammalian In Vitro System." Ph.D. in Biochemistry and Biophysics
  • Jongsma, Candice G., 2008. “Investigating Cotranslational Integration of a Multi-Spanning Membrane Protein into the Endoplasmic Reticulum Membrane.”  Ph.D. in Chemistry.

M.S. Theses Directed by Dr. Johnson

University of Oklahoma

  • Krieg, Ute C., 1984. "An Examination of the Interaction of Blood Coagulation Factor Va with Phospholipid Vesicles Using Lipophilic Photoreagents."
  • Janiak, Fabiola, 1985. "Fluorescence-Detected Interactions of Various Aminoacyl-tRNAs with Escherichia coli Elongation Factor Tu."
  • Tober, Carsten, 1992. "Photocrosslinking Studies of the Environment of the Stop-Transfer Sequence of Transmembrane Proteins during Integration."

Texas A&M University

  • Su, Bixia, 1998. "Tissue Factor Positions and Maintains the Factor VIIa Active Site Far Above the Membrane Surface Even in the Absence of the Factor VIIa Gla Domain." M.S. in Chemistry.
  • Cargill, Holly B., 2006. "Assaying Protein Import into Mitochondria using Fluorescence Spectroscopy." M.S. in Biochemistry and Biophysics.
  • (more directed dissertations and theses...)

Professional Societies

  • American Association for the Advancement of Science
  • American Chemical Society
  • American Society for Biochemistry and Molecular Biology
  • American Society for Cell Biology
  • Biophysical Society
  • Biophysical Society, Biological Fluorescence Subgroup