Publications

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Pariyarath, R., Wang, H., Aitchison, J. D., Ginsberg, H. N., Johnson, A. E., and Fisher, E. A. (2001) "Co-translational Interactions of Apoprotein B with the Ribosome and Translocon during its Assembly with Lipids in the Endoplasmic Reticulum or its Targeting to Proteasomal Degradation in the Cytosol," Journal of Biological Chemistry 276, 541-550.
Tweten, R. K., Parker, M. W., and Johnson, A. E. (2001) "The Cholesterol-Dependent Cytolysins," in Pore-Forming Toxins (van der Goot, G., ed.), pp. 15-33, Springer-Verlag, Heidelberg.
Hotze, E. M., Kubalek, E., Rossjohn, J., Parker, M., Johnson, A. E., and Tweten, R. K. (2001) "Arresting Pore Formation of a Cholesterol-Dependent Cytolysin by Disulfide Trapping Synchronizes the Insertion of the Transmembrane β-Sheet from a Prepore Intermediate," Journal of Biological Chemistry 276, 8261-8268.
Vidugiriene, J., Vainauskas, S., Johnson, A. E., and Menon, A. K. (2001) "Endoplasmic Reticulum Proteins Involved in Glycosylphosphatidylinositol Anchor Attachment: Photocrosslinking Studies in a Cell-Free System," European Journal of Biochemistry 268, 2290-2300.
Heuck, A.P., Tweten, R.K., and Johnson, A.E. (2001) "β-Barrel Pore-Forming Toxins: Intriguing Dimorphic Proteins," Biochemistry 40, 9065-9073.
Nilsson, I., Ohvo-Rekilä, H., Slotte, J.P., Johnson, A.E., and von Heijne, G.* (2001) "Inhibition of Protein Translocation across the ER Membrane by Sterols," Journal of Biological Chemistry 276, 41748-41754.
Jensen, R.E., and Johnson, A.E. (2001) "Opening the Door to Mitochondrial Protein Import," Nature Structural Biology 8, 1008-1010.
Johnson, A.E., Chen, J.-C., Flanagan, J.J., Miao, Y., Shao, Y., Lin, J., and Bock, P.E. (2001) "Structure, Function, and Regulation of Free and Membrane-bound Ribosomes: The View from their Substrates and Products," in The Ribosome, Cold Spring Harbor Symposia on Quantitative Biology 66, 531-541.
Heuck, A.P., and Johnson, A.E. (2002) "Pore-Forming Protein Structure Analysis in Membranes Using Multiple Independent Fluorescence Techniques," Cell Biochemistry and Biophysics 36, 89-101.
Haigh, N. G., and Johnson, A. E. (2002) "A New Role for BiP: Closing the Aqueous Translocon Pore during Protein Integration into the ER Membrane," Journal of Cell Biology 156, 261-270.
Haigh, N.G., and Johnson, A.E.* (2002) "Protein Sorting at the Membrane of the Endoplasmic Reticulum," in Protein Targeting, Transport, and Translocation (Dalbey, R., and von Heijne, G., eds.), pp. 74-106, Academic Press, London.
Hotze, E.M., Heuck, A.P., Czajkowsky, D., Shao, Z., Johnson, A.E., and Tweten, R.K. (2002) "Monomer-Monomer Interactions Drive the Prepore to Pore Conversion of a β-Barrel-Forming Cholesterol-Dependent Cytolysin," Journal of Biological Chemistry 277, 11597-11605.
Nilsson, I., Johnson, A.E., and von Heijne, G. (2002) "Cleavage of a Tail-Anchored Protein by Signal Peptidase," FEBS Letters, 516, 106-108.
Ramachandran, R., Heuck, A.P., Tweten, R.K., and Johnson, A.E.* (2002) "Structural Insights into the Membrane-Anchoring Mechanism of a Cholesterol-Dependent Cytolysin," Nature Structural Biology 9, 823-827.
Daniels, R., Kurowski, B., Johnson, A.E., and Hebert, D.N. (2003) "N-linked Glycans Direct the Co-Translational Folding Pathway of Influenza Hemagglutinin," Molecular Cell 11, 79-90.
Mori, H., Tsukazaki, T., Masui, R., Kuramitsu, S., Yokoyama, S., Johnson, A.E., Kimura, Y., Akiyama, Y., and Ito, K. (2003) "Fluorescence Resonance Energy Transfer Analysis of Protein Translocase: SecYE from Thermus thermophilus HB8 Forms a Constitutive Oligomer in Membranes,' Journal of Biological Chemistry 278, 14257-14264
Flanagan, J.J., Chen, J.-C., Miao, Y., Shao, Y., Lin, J., Bock, P.E., and Johnson, A.E. (2003) "SRP Binds to Ribosome-bound Signal Sequences with Fluorescence-detected Subnanomolar Affiity that Does Not Diminish as the Nascent Chain Lengthens," Journal of Biological Chemistry 278, 18628-18637.
Nilsson, I., Kelleher, D.J., Miao, Y., Shao, Y., Kreibich, G., Gilmore, R., von Heijne, G., and Johnson, A.E. (2003) "Photocrosslinking of Nascent Chains to the STT3 Subunit of the Oligosaccharyltransferase Complex," Journal of Cell Biochemistry 161, 715-725.
Leach, J.K., O'Rear, E.A., Patterson, E., Miao, Y., and Johnson, A.E. (2003) "Accelerated Thrombolysis in a Rabbit Model of Carotid Artery Thrombosis with Liposome-Encapsulated and Microencapsulated Streptokinase," Thrombosis and Haemostasis 90, 64-70.
Nilsson, I., Johnson, A.E., and von Heijne, G. (2003) "How Hydrophobic is Alanine?" Journal of Biological Chemistry 278, 29389-29393.
Heuck, A.P., Tweten, R.K., and Johnson, A.E. (2003) "Assembly and Topography of the Prepore Complex in Cholesterol-Dependent Cytolysins," Journal of Biological Chemistry 278, 31218-31225.
Johnson, A.E. (2003) "Maintaining the Permeability Barrier during Protein Trafficking at the ER Membrane," Biochemical Society Transactions 31, 1227-1231.
McCormick, P.J., Miao, Y., Shao, Y., Lin, J., and Johnson, A.E. (2003) "Cotranslational Protein Integration into the ER Membrane Is Mediated by the Binding of Nascent Chains to Translocon Proteins," Molecular Cell 12, 329-341.
Giddings, K. S., Johnson, A. E., and Tweten, R. K. (2003) "Redefining Cholesterol's Role in the Mechanism of the Cholesterol-Dependent Cytolysins," Proceedings of the National Academy of Sciences (USA) 100, 11315-11320.
Johnson, A.E. and Jensen, R.E. (2004) "Barreling through the Membrane," Nature Structural & Molecular Biology 11, 113-114.
Woolhead, C., McCormick, P.J., and Johnson, A.E. (2004) "Nascent Membrane and Secretory Proteins Differ in FRET-Detected Folding Far Inside the Ribosome and in their Exposure to Ribosomal Proteins," Cell 116, 725-736.
Alder, N.N. and Johnson, A.E. (2004) "Cotranslational Membrane Protein Biogenesis at the Endoplasmic Reticulum," Journal of Biological Chemistry 279, 22787-22790.
Ramachandran, R., Tweten, R. K., and Johnson, A. E. (2004) “Membrane-Dependent Conformational Changes Initiate Cholesterol-Dependent Cytolysin Oligomerization and Intersubunit β-Strand Alignment,” Nature Structural & Molecular Biology 11, 697-705.
Johnson, A. E. (2004) “Functional Ramifications of FRET–Detected Nascent Chain Folding Far Inside the Membrane–Bound Ribosome,” Biochemical Society Transactions 32, 668-672.
Saksena, S., Shao, Y., Braunagel, S., Summers, M. D., and Johnson, A. E. (2004) "Cotranslational Integration and Initial Sorting at the ER Translocon of Proteins Destined for the Inner Nuclear Membrane," Proceedings of the National Academy of Sciences (USA) 101, 12537-12542.
Zhang, Z., Lapolla, S. M., Annis, M. G., Truscott, M., Roberts, G. J., Miao, Y., Shao, Y., Tan, C., Peng, J., Johnson, A. E., Zhang, X. C., Andrews, D. W., and Lin, J. (2004) “Bcl-2 Homodimerization Involves Two Distinct Binding Surfaces, a Topographic Arrangement that Provides an Effective Mechanism for Bcl-2 to Capture Activated Bax,” Journal of Biological Chemistry 279, 43920-43928.
Huck, L., Scherrer, A., Terzi, L., Johnson, A. E., Bernstein, H. D., Cusack, S., Weichenrieder, O., and Strub, K. (2004) “Conserved Tertiary Base Pairing Ensures Proper RNA Folding and Efficient Assembly of the Signal Recognition Particle Alu Domain,” Nucleic Acids Research 32, 4915-4924,.
Johnson, A. E. (2005) "The Co-Translational Folding and Interactions of Nascent Protein Chains: A New Approach Using Fluorescence Resonance Energy Transfer," FEBS Letters 579, 916-920. Nobel Symposium special issue.
Alder, N.N., Shen, Y., Brodsky, J.L., Hendershot, L.M., and Johnson, A.E. (2005) "The Molecular Mechanisms Underlying BiP-Mediated Gating of the Sec61 Translocon of the Endoplasmic Reticulum," Journal of Biological Chemistry 168, 389-399.
Heuck, A.P. and Johnson, A.E. (2005) "Membrane Recognition and Pore Formation by Bacterial Pore-Forming Toxins" in Protein-Lipid Interactions (L.K. Tamm, editor), Wiley-VHC Weinheim, pp. 163-186.
Giddings, K.S., Johnson, A.E., and Tweten, R.K. (2005) "Perfringolysin O and Intermedilysin: Mechanisms of Pore Formation by the Cholesterol-Dependent Cytolysins," in The Comprehensive Sourcebook of Bacterial Protein Toxins, Third Edition (J.E. Alouf and M.R. Popoff, editors), Academic Press, Oxford, England, pp. 671-679.
Ramachandran, R., Tweten, R.K., and Johnson, A.E. (2005) "The Domains of a Cholesterol-Dependent Cytolysin Undergo a Major FRET-Detected Rearrangement during Pore Formation," Proceedings of the National Academy of Sciences (USA) 102, 7139-7144.
Saurí, A., Saksena, S., Salgado, J., Johnson, A. E., and Mingarro, I. (2005) "Double-Spanning Plant Viral Movement Protein Integration into the ER Membrane Is SRP-Dependent, Translocon-Mediated, and Concerted," Journal of Biological Chemistry 280, 25907-25912.
Etchells, S.A., Meyer, A.S., Yam, A.Y., Roobol, A., Miao, Y., Shao, Y., Carden, M.J., Skach, W.R., Frydman, J., and Johnson, A.E. (2005) "The Cotranslational Contacts between Ribosome-Bound Nascent Polypeptides and the Subunits of the Hetero-Oligomeric Chaperonin TRiC Probed by Photocrosslinking," Journal of Biological Chemistry 280, 28118-28126.
Johnson, A.E. (2005) "Fluorescence Approaches for Determining Protein Conformations, Interactions and Mechanisms at Membranes," Traffic 6, 1078-1982.
Karamyshev, A.L., and Johnson, A.E. (2005) "Selective SecA Association with Signal Sequences in Ribosome-Bound Nascent Chains: A Potential Role for SecA in Ribosome Targeting to the Bacterial Membrane," Journal of Biological Chemistry 280, 37930-37940.
Sadlish, H., Pitonzo, D., Johnson, A.E., and Skach, W.R. (2005) "Sequential Triage of Transmembrane Segments by Sec61α During Biogenesis of a Native Multispanning Membrane Protein," Nature Structural & Molecular Biology 12, 870-878.
Karamyshev, A. L., Kelleher, D. J., Gilmore, R., Johnson, A. E., von Heijne, G., and Nilsson, I. (2005) "Mapping the Interaction of the STT3 Subunit of the Oligosaccharyltransferase Complex with Nascent Polypeptide Chains," Journal of Biological Chemistry 280, 40489-40493.
Tomic, S., Johnson, A. E., Hartl, F. U., and Etchells, S. A. (2006) "Exploring the Capacity of Trigger Factor to Function as a Shield for Ribosome-Bound Polypeptide Chains," FEBS Letters 580, 72-76.
Seo, J., Barhoumi, R., Johnson, A. E., Lupton, J. R., and Chapkin, R. S. (2006) "Docosahexaenoic Acid Selectively Inhibits Plasma Membrane Targeting of Lipidated Proteins," FASEB Journal 20, 770-772.
Frankel, D. J., Pfeiffer, J. R., Surviladze, Z., Johnson, A. E., Oliver, J. M., Wilson, B. S., and Burns, A. R. (2006) "Revealing the Topography of Membrane Domains by Combined AFM/Fluorescence Imaging," Biophysical Journal 90, 2404-2413.
Araç, D., Chen, X., Khant, H. A., Ubach,J., Ludtke, S. J., Kikkawa, M., Johnson, A. E., Chiu, W., Südhof, T. C., and Rizo, J. (2006) "Membrane-Membrane Proximity Induced by Ca²⁺-Dependent Multivalent Binding of Synaptotagmin 1 to Phospholipids," Nature Structural & Molecular Biology 13, 209-217.
Saksena, S., Summers, M. D., Burks, J. K., Johnson, A. E., and Braunagel, S. C. (2006) “Importin α-16: a Translocon-Associated Protein that May Facilitate Sorting of Integral Membrane Proteins to the Nuclear Envelope,” Nature Structural & Molecular Biology 13, 500-508.
Woolhead, C. A., Johnson, A. E., and Bernstein, H. D. (2006) “Translation Arrest Requires Two-Way Communication between a Nascent Polypeptide and the Ribosome,” Molecular Cell 22, 587-598.
Davis, A. J., Alder, N. N., Jensen, R. E., and Johnson, A. E. (2007) “The Tim9p/10p and Tim8p/13p Complexes Bind to Specific Sites on Tim23p during Mitochondrial Protein Import,” Molecular Biology of the Cell 18, 475-486.
Saurí, A., McCormick, P. J., Johnson, A. E., and Mingarro, I. (2007) “Sec61α and TRAM Are Sequentially Adjacent to a Nascent Viral Membrane Protein during Its ER Integration,” Journal of Molecular Biology 366, 366-374.
Lakshmipathy, S. K., Tomic, S, Kaiser, C. M., Chang, H.-C., Genevaux, P., Georgopoulous, C., Barral, J. M., Johnson, A. E., Hartl, F. U., and Etchells, S. A. (2007) “Identification of Nascent Chain Interaction Sites on Trigger Factor,” Journal of Biological Chemistry 282, 12186-12193.
Wahlman, J., DeMartino, G. N., Skach, W. R., Bulleid, N. J., Brodsky, J. L., and Johnson, A. E. (2007) “Real-time Fluorescence Detection of ERAD Substrate Retro-translocation in a Mammalian In Vitro System,” Cell 129, 943-955
Soltani, C. E., Hotze, E. M., Johnson, A. E., and Tweten, R. K. (2007) “Specific Protein-Membrane Contacts Are Required for Prepore and Pore Assembly by a Cholesterol-Dependent Cytolysin,” Journal of Biological Chemistry 282, 15709-15216
Heuck, A. P., Savvo, C. G., Holzenburg, A., and Johnson, A. E. (2007) “Conformational Changes that Effect Oligomerization and Initiate Pore Formation Are Triggered throughout Perfringolysin O upon Binding to Cholesterol,” Journal of Biological Chemistry 282, 22629-22637..
Lundin, C., Johansson, S., Johnson, Arthur E., Näslund, J., von Heijne, G., Nilsson, IM. (2007) “Stable Insertion of Alzheimer Ab Peptide into the ER Membrane Strongly Correlates with its Length,” FEBS Letters 581, 3809-3813.
Soltani, C. E., Hotze, E. M., Johnson, A. E., and Tweten, R. K. (2007) “Structural Elements of the Cholesterol-Dependent Cytolysins that Are Responsible for their Cholesterol-Sensitive Membrane Interactions,” Proceedings of National Academy of Science (USA) 104, 20226-20231.
Alder, N. N., Sutherland, J., Buhring, A. I., Jensen, R. E., and Johnson, A. E. (2008) “Quaternary Structure of the Mitochondrial TIM23 Complex Reveals Dynamic Association between Tim23p and Other Subunits,” Molecular Biology of the Cell 19, 159-170.
Bowden, M. G., Heuck, A. P., Ponnuraj, K., Kolosova, E., Choe, D., Gurusiddappa, S., Narayana, S. V. L., Johnson, A. E., and Höök, M. (2008) “Evidence for the “Dock, Lock, and Latch” Ligand Binding Mechanism of the Staphylococcal MSCRAMM SDRG,” Journal of Biological Chemistry 283, 638-647
Nelson, L., Johnson, A. E., and London, E. (2008) “How the Interaction of Perfringolysin O with Membranes Is Controlled by Sterol Structure, Lipid Structure, and Physiological Low pH: Insights into the Origin of Perfringolysin O-Lipid Raft Interaction,” Journal of Biological Chemistry 283, 4632-4642
Lakkaraju, A. K. K., Mary, C., Scherrer, A., Johnson, A. E., and Strub, K. (2008) “SRP Maintains Nascent Chains Translocation-Competent by Slowing Translation Rates to Match Limiting Numbers of Targeting Sites,” Cell 133, 440-451.
Alder, N. N., Jensen, R. E., and Johnson, A. E. (2008) “Fluorescence Mapping of Mitochondrial TIM23 Complex Reveals a Water-Facing, Substrate-Interacting Helix Surface,” Cell 134, 439-450.
Daniel, C., Conti, B., Johnson, A. E., and Skach, W. R. (2008) “Control of Translocation through the Sec61 Translocon by Nascent Polypeptide Structure within the Ribosome,” Journal of Biological Chemistry 283, 20864-20873.
Saksena, S., Wahlman, J., Teis, D., Johnson, A. E., and Emr, S. D. (2009) “Functional Reconstitution of ESCRT-III Assembly and Disassembly,” Cell 136, 97-109.
Pitonzo, D., Yang, Z., Rusterholtz, K., Johnson, A. E., and Skach, W. R. (2009) “Sequence-Specific Retention and Regulated Release of a Nascent Translocating Polypepetide by the ER Sec61 Translocon,” Molecular Biology of the Cell 20, 685-698.
Berndt, U., Oellerer, S., Zhang, Y., Johnson, A. E., and Rospert, S. (2009) “A Signal-Anchor Sequence Stimulates Signal Recognition Particle Binding to Ribosomes from Inside the Exit Tunnel,” Proceedings of the National Academy of Sciences (USA) 106, 1398-1403.
Saurí, A., Tamborero, S., Martínez-Gil, L., Johnson, A. E., and Mingarro, I. (2009) “Viral Membrane Protein Topology Is Dictated by Multiple Determinants in Its Sequence,” Journal of Molecular Biology 387, 113-128.
Mayerhofer, P. U., Cook, J. P., Wahlman, J., Pinheiro, T. T. J., Moore, K. A. H., Lord, J. M., Johnson, A. E., and Roberts, L. M. (2009) “Ricin A-Chain Insertion into ER Membranes Is Triggered by a Temperature Increase to 37°C,” Journal of Biological Chemistry 284, 10232-10242.
Flanagan, J.J. Tweten, R.K., Johnson, A.E., and Heuck, A.P. (2009) "Cholesterol Exposure at the Membrane Surface is Necessary and Sufficient to Trigger Perfringolysin O Binding," Biochemistry 48, 3977-3987.
Johnson, A.E. (2009) "The Structural and Functional Coupling of Two Molecular Machines, the Ribosome and the Translocon," Journal of Cell Biology 185, 765-767.
Farrad, A. J., LaChapelle, S., Hotze, E., Johnson, A. E., and Tweten, R. K. (2010) "Only Two Amino Acids Are Essential for Cytolytic Toxin Recognition of Cholesterol at the Membrane Surface," Proceedings of the National Academy of Sciences (USA) 107, 4341-4346.
Mary, C., Scherrer, A., Huck, L. Lakkaraju, A. K. K., Thomas, Y., Johnson, A. E., and Strub, K. (2010) "Residues in SRP9/14 Essential for Elongation Arrest Activity of the Signal Recognition Particle Define a Positively Charged Functional Domain on One Side of the Protein," RNA 16, 969-979.
Martinez-Gil, L., Johnson, A. E., and Mingarro, I. (2010) "Membrane Insertion and Biogenesis of the Turnip Crinkle Virus p9 Movement Protein," Journal of Virology 84, 5520-5527.
Zhang, Z., Zhu, W. Lapolla, S. M., Miao, Y., Shao, Y., Falcone, M., Johnson, A. E., Zhang, X. C., Andrews, D. W., and Lin, J. (2010) "Bax Forms an Oligomer by a Sequential Mechanism Utilizing Separate, Yet Interdependent, Surfaces," Journal of Biological Chemistry 285. 17614-17627.
Gubbens, J., Kim, S. J., Yang, Z., Johnson, A. E., and Skach, W. R. (2010) "Efficient In Vitro Incorporation of Non-natural Amino Acids into Protein Using tRNA^Cys-derived Opal, Ochre, and Amber Supressor tRNAs," RNA 16, 1660-1672.
Devaraneni, P., Conti, B., Matsumura, Y., Yang, Z., Johnson, A. E., and Skach, W. R. (2011) “Head-First Insertion and Inversion if a Type II Signal Anchor Occurs within a Proteinaceous Environment of the Ribosome-Sec61 Complex, in press.