Lab Facilities
The Johnson group occupies a contiguous 7-room, 3,723 sq. ft. laboratory complex that contains three hoods and the usual utilities. One room includes a 180 sq. ft. walk-in cold room and a 109 sq. ft. dark room for photocrosslinking and photosensitive preparations. A spectroscopy lab houses the four spectrofluorimeters and laser.
Major Research Equipment Located in the Johnson Labs
- 3 -SLM 8100 photon-counting spectrofluorimeters
- ISS K2-002 multifrequency cross-correlation phase and modulation fluorimeter
- Coherent Innova 400-15/3 argon ion laser
- Bio-Rad Molecular Imager FX with dedicated microcomputer
- Beckman 640 UV-Vis absorption spectrophotometer
- Beckman LS-6500 liquid scintillation counter
- Molecular Devices Vmax kinetic microplate reader interfaced to a dedicated microcomputer
- Perkin-Elmer 9700 PCR System
- Perkin-Elmer 480 DNA thermal cycler
- Beckman L7-65 preparative ultracentrifuge with five different rotors
- Beckman J2-HS high-speed centrifuge with three different rotors
- Beckman Optima TL table-top ultracentrifuge with three different rotors
- Beckman Airfuge with two different rotors
- Amersham-Pharmacia ÄKTA chromatography system with dedicated microcomputer
- 2 - Pharmacia FPLC systems
- Gilson HPLC system with a dual wavelength UV-Vis detector, a fluorescence flow monitor, and a dedicated microcomputer
- Pharmacia Phast-Gel system
- Oriel mercury arc lamp and power supply for photocrosslinking
- 200-watt Heat Systems sonicator, cup horn, and microprobe
- Three -95°C ultracold freezers
- New Brunswick G24 incubator shaker (capacity = 3 L)
- New Brunswick G25 incubator shaker (floor model; capacity = 24 L)
- Thermo Spectronic French pressure cell press
- Two high-voltage paper electrophoresis apparati
Computers
We have 4 free-standing PC and Apple microcomputers for data analysis, data presentation, and word processing. Each of these computers is linked to the University network. In addition, 6 microcomputers are dedicated to particular instruments. All computers are networked to 2 laser printers, a color ink-jet printer, and a Kodak 8650 digital printer. In addition, we have a Kodak DC290 Digital camera, a Pentium III 700 laptop PC microcomputer, and a portable projector for PowerPoint presentations.
