Sudhiranjan Gupta, Ph.D.
TAMHSC
COMClinical DepartmentsInternal MedicineMolecular CardiologyFaculty and StaffSudhiranjan Gupta, Ph.D.
Assistant Professor
Department of Internal Medicine
1901 South 1st Street, Building 205
Temple,
Texas 76504
Phone: 254-743-2465
Fax: 254-743-0165
Email: sgupta@medicine.tamhsc.edu
Curriculum Vitae
(PDF)
Education and Post-Graduate Training
M. S., Visva Bharati University, Santiniketan, India, 1990
Ph.D., Molecular Biology, Bose Institute, Calcutta, India, 1991-1997
Post Doctoral Fellow, Weizmann Institute of Science, Rehovot, Israel, 1997-1998
Post Doctoral Fellow, Molecular Cardiology, Cleveland Clinic, Cleveland, Ohio, 1998-2002
Research Associate, Molecular Cardiology, Cleveland Clinic, Cleveland, Ohio, 2002-2005
Project Scientist, Molecular Cardiology, Cleveland Clinic, Cleveland, Ohio, 2005-2009gp
Teaching Interests
Cleveland Clinic, Lerner College of Medicine at Case, 2006-2008
Research Interests
Current program:
Role of NF-KB in extracellular matrix protein remodeling in cardiac hypertrophy and heart failure; role of thymosin ß4 and integrin link kinase (ILK) in cardiac remodeling and role of ILK and NF-KB in pulmonary arterial hypertension.
Role of NF-KB in extracellular matrix protein remodeling in cardiac hypertrophy and heart failure- Excessive collagen deposition and cardiac fibrosis contributes to remodeling of the heart in various stages of cardiac hypertrophy and the subsequent transition to heart failure. The molecular changes in the extracellular matrix and collagen type switch are hallmarks of the remodeling process. We have recently shown that NF-KB, a ubiquitous inducible transcription factor, plays a major role in cardiac hypertrophy, heart failure and remodeling. However, the detailed understanding of the role of NF-KB in collagen remodeling (cardiac) is not known and the intermediate signaling molecules that play a role to bridge the NF-KB activation associated with change in collagen synthesis is yet to be explored. We are currently exploring the underlying molecular mechanism of ECM regulation by NF-KB utilizing both in vitro (cardiac myocytes and fibroblasts) and in vivo systems (transgenic mice).
Role of thymosin ß4 and integrin link kinase (ILK) in cardiac remodeling - ILK is an ankyrin bearing protein, serves as a molecular scaffold at the sites of integrins, contributes to regulation of cellular adhesion, migration and differentiation after a variety of stimuli. In cardiomyocytes, integrins are also thought to be a transducer of biochemical stress into a compensatory growth program on the basis of their role in liking extracelluar matrix with intracellular signaling pathways affecting growth and survival. Deregulation of the ILK/PINCH interaction causes severe cellular defects but the clinical importance and molecular details of such interaction remain undetermined in failing human hearts. Thymosin ß4, a monomeric G-actin protein, has been shown to play an important role in many diverse fields including promoting angiogenesis suppressing inflammation and apoptosis, improve cardiomyocytes recovery after hypoxia via activation of ILK. We are currently investigating how thymosinß4 or related pathway members, protect tissues from cardiac damage utilizing both in vitro (oxidative stress) and in vivo systems (transgenic mice).
Role of ILK and NF-kB in pulmonary arterial hypertension - Pulmonary hypertension is a prominent feature of interstitial lung diseases (ILD), including systemic sclerosis, granulomatous diseases, and idiopathic pulmonary fibrosis (IPF). Despite major advances made in the understanding of the pathogenesis of pulmonary artery hypertension (PAH), the precise molecular mechanism remains elusive. Both inflammation and oxidant stress have been implicated in the pathogenesis of PAH. We are interested to examine the involvement of ILLK and NF-KB in restoring the vascular tone utilizing in vitro (pulmonary artery smooth muscle or endothelial cells) and in vivo model systems (monocrotaline induced PH mice model). Additionally, we are interested to evaluate the effects of targeted drugs in restoring normal endothelial-dependent vasodilative response.
Selected Publications
Fukuda K, Gupta S, Chen K, Wu, C, Qin J. The pseudoactive site of ILK is essential for its binding to alpha-Parvin and localization to focal adhesions. Molecular Cell, 36:8190830, 2009. Selected for Faculty of 1000 Biology.
Gupta A, Gupta S, Young D, Sen S. Ultrastructural changes during progression of hypertrophy to heart failure in myotrophin over-expressed transgenic mice. Lab Investigation, in press, 2009.
Gupta S, Maitra R, Young D, Gupta A, Sen S. Ablation of myotrophin gene by RNA interference shows attenuation of cardiac hypertrophy. Am J Physiol 2975:H627-H636, 2009.
Ithychanda S, Das M, Ma Y, Ding K, Wang X, Gupta S, Wu C, Plow E, Qin J. Migfilin: a molecular switch in regulation of integrin activation. J Biol chem 284:4713-4722, 2009.
Young D, Popovic Z, Jones K and Gupta S. Blockade of NF-kB using Ikb dominant negative mice ameliorates cardiac hypertrophy in myotrophin overexpressed transgenic mice. J Mol Biol. 381:559-568, 2008.
Das B, Gupta S, Vasanji A, Misra S, Sen S. Nuclear co-translocation of myotrophic and p65 stimulates myocyte growth: regulation by myotrophin hairpin loops. J. Biol Chem. 283:2794F-27956, 2008.
Gupta S, Young D, Maitra R, Gupta A, Zoran P, Yoong S, Mahajan A, Wang Q and Sen S. Prevention of cardiac hypertrophy and heart failure by silencing of NF-kB. J Mol Biol. 375:637-649, 2008.
Sivakumar P, Gupta S, Sarkar S, Sen S. Upregulation of lysyl oxidase and MMP's during cardiac remodeling in human dilated cardiomyopathy. Mol Cell Biochem. 307 (1-2):159-167, 2008.
Reddy K, Fox J, Price M, Kulkarni S, Gupta S, Das B, Smith D. Nuclear localization of myomesin-1: possible functions. J Muscle Res Cell Motil. 29(1):1-8, 2008.
Gupta S, Das B, Sen S. Cardiac hypertrophy: Mechanism and therapeutic opportunities. Antioxidants and Redox Signaling. 6:623-652, 2007.
Gupta S, Sen S. Molecular mechanism of cardiac hypertrophy and heart failure in animal models. Methods in Molecular Medicine/Cardiovascular Disease. 129:97-114, 2006.
Gupta S, Sen S. The role of NF-kb signaling cascade and NF-kB-targeted genes in failing human hearts. J Mol Med. 83:993-1004, 2005.
Adhikary A, Gupta S, Saad Y, Sil P. and Sen S. Characterizations and functional significance of myotrophin: A gene with multiple transcripts. Gene. 353:31-40, 2005.
Gupta S, Young D and Sen S. Inhibition of NF-kb induces regression of cardiac hypertrophy, independent of blood pressure control in spontaneously hypertensive rats. Am J Physiol Heart & Circ. 289: H20-H29, 2005.
Gupta S. Cardiac hypertrophy and heart failure: Current perspective in gene expression profiling and signal transduction. J Med Biochem. 1:3-9, 2004.
Sil P, Gupta S,Young D, Sen S. Regulation of myotrophin gene by pressure overload and stretch. Mol Cell Biochem. 262:79-89, 2004.
Sarkar S, Leamann DW, Gupta S, Sil P, Young D, Morehead A, Mukherjee D, Ratliff N, Sun Y, Rayborn M, Hollyfield H , Sen S. Cardiac overexpression of myotrophin triggers myocardial hypertrophy and heart failure in transgenic mice. J Biol Chem. 279:20422-20434, 2004.
Gupta S, Purcell N, Lin A, Sen S. Activation of nuclear factor kB is necessary for myotrophin-induced cardiac hypertrophy. J Cell Biol. 159:1019-1028, 2002.
Gupta S, Sen S. Myotrophin: kB DNA interaction in the initiation process of cardiac hypertrophy. BBA. 1589(3):247-260, 2002.
Mitra S, Timur A, Gupta S, Qang Q, Sen S. Assignment of myotrophin to human chromosome band 7qee-35 by in-situ hybridization. Cytogenetics and Cell Genetics. 93:151-152, 2001.
Chackraborti P, Gupta S, Gupta-Bhattacharya S, Chanda S. Airborne pollen of Borassus flabellifer: quantification of allergen and antigen. Aerobiologia. 15:49-55, 1999.
Chackraborti P, Chowdhuri I, Gupta-Bhattacharya S, Gupta S, Ghosh B. Clinico-immunological studies on Phoenix sylvestris Rosb. Pollen: an aeroallergen from India. Allergy. 54(9):985-989, 1999.
Bose A, Tiwari B, Chattaopadhyay, Gupta S, Ghosh B. Thermal stress induced different response on Rubisco degradation in heat sensitive and heat tolerant rice cultivars. Physiologia Plantarum. 105:89-94, 1999.
Gupta S, Chatterjee M, Chatterjee P, Dibyendu N, Sengupta D, Ghosh B. Expression of abscisic acid responsive element (Em1a) protein in salt tolerant. Indica Rice (Oryza sativa L cv Pkkkali). Plant Molecular Biology. 317:629-637, 1998.
Chatterjee M, Gupta S, SenGupta D, Ghosh B. Expression of rginine decarboxylase in seedlings on Indica Rice (Oryza sativa L( cultivars as affected by salinity stress. Plant Molecular Biology. 34:477-483, 1997.
National Service/Recognition
Recipient of Bernadine Healy Award, Department of Molecular Cardiology, Cleveland Clinic Foundation, 2004.
Recipient of Travel Award, (for best abstract) from SHR-North America Section, 2004 for attending the meeting at Cancun, Mexico.
Recipient of Irvine Page Merit Award, Department of Molecular Cardiology, Cleveland Clinic Foundation for publishing Best Paper, 2002-2003.
Selected as one of the four finalists in Young Investigators Award, International Society for Heart Research (ISHR), North America Section, 2003, Mystic, Connecticut, USA.
Recipient of scholarship from Department of Health and Human Services, NIH, NIC: Grant #1R13 HL 69887-01 for presenting Best Poster in the meeting entitled "Molecular Biology of Heart", Keystone Symposium, 2002, Keystone, Colorado, USA.
National Qualification in Graduate Aptitude Test in Engineering (GATE), Government of India, 1990.
Member Indian Journal of Medical Biochemistry, 2003-present.
Grant Reviewer - American Heart Association
