Dr. Prabhakar Tiwari
Postdoctoral Research Associate
Scott & White Memorial Hospital and Clinic and
The Texas A&M Health Science Center College of Medicine
Phone: 254-743-1215
Fax: 254-743-0247
Email: ptiwari@medicine.tamhsc.edu

Education
2000 - B.Sc., Botany, Zoology and Chemistry from Awadhesh Pratap Singh (A. P. S.)
University, Rewa, India
2002 - M.Sc., Biotechnology from Awadhesh Pratap Singh (A. P. S.) University, Rewa,
India
2011 - Ph.D., Neuro-molecular biology from Awadhesh Pratap Singh (A. P. S.) University, Rewa, India

Summary of Research Interests
Our research interest is the development of vaccine against the cancer by the fusion of receptors with heat shock proteins (HSP) using Baculovirus Expression Vector Systems (BEVS) technology. Our long-term goal is to determine the efficacy of the HSP chimeras with fusion of receptors in an animal model and to elucidate the mechanism by which the HSP-based vaccination enhances anti-tumor immunity, which ultimately inhibits tumor growth. By this technology, we would capitalize on the strategy of harnessing the low cost effective therapy in the cure of cancer.

In turn, we would make it feasible to augment the potency of current thermal therapy protocols and HSP-based therapies for a variety of human diseases including cancers, neuro-degeneration, cardiovascular disease, autoimmunity and aging. Finally, it is expected that these results will fundamentally advance the field of HSP-based immunobiology.

Publications

• Rekha Dwivedi, Prabhakar Tiwari, Nasim Mansoori, Rizwan Alam, UK Chauhan , Manjari Tripathi. Association of cytokine inflammatory genes (IL1-β-511-C>T, TNF-α -308-G>A and IL6-174-G>C) polymorphism with epilepsy patients in north Indian population (In Review).
• Rakesh kumar Singh, Prabhakar Tiwari, Suman Gupta, Sudhir Sahdev, Renu Malik, Sunanda G. Dastidar, Abhijit Ray. A Fluorescent based enzyme assay for recombinant human lipoxygenase enzyme isoforms (In Review).
• Sudhir Sahdev, Prabhakar Tiwari, Shalini Saini, Sanatan Upmanyu, Sulakshana Jain, Suman Gupta, Lalitha Vijayakrishnan, Manish Diwan, Sunanda G. Dastidar, Jyoti Paliwal, Abhijit Ray, Pradip Kumar Bhatnagar and Kulvinder Singh Saini. Improved Baculovirus-Insect Cell Culture Media for Enhanced Production of Active Eukaryotic Proteins (In Review).
• Sundaram S, Tiwari P, Saini S, Kant R, Davis JA, Sahdev S, Saini KS. 2010. Baculovirus expression system: an alternative for producing catalytically active human PTP-1B. Mol Biol (Mosk). May-Jun; 44(3):535-40.
• Tiwari P, Saini S, Upmanyu S, Benjamin B, Tandon R, Saini KS, Sahdev S. 2010. Enhanced expression of recombinant proteins utilizing a modified baculovirus expression vector. Mol Biotechnol. Sep; 46(1):80-9.
• Sahdev S, Saini S, Tiwari P, Saxena S, Singh Saini K. 2007. Amplification of GC-rich genes by following a combination strategy of primer design, enhancers and modified PCR cycle conditions. Mol Cell Probes. Aug; 21(4):303-7.