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College of Medicine Cell Analysis Facility

About

The College of Medicine Cell Analysis Facility (COM-CAF) at the Texas A&M Health Science Center (TAMHSC) provides cell analysis and cell sorting services for research in the TAMHSC and TAMU community and is managed by the Department of Microbial Pathogenesis and Immunology.

The mission of the COM-CAF is

  • To promote research and training for cell-based assays at the highest level of quality and rigor
  • To bring new developments in flow cytometric instrumentation, technique and analysis for preclinical use in the TAMHSC and TAMU research community
  • To support the productivity and effectiveness of research by promoting high-content data acquisition, ensuring the highest data quality and consistency, carrying out efficient cell sorting, providing reliable data management, educating and training researchers and consulting in experiment design and evaluation.

Major applications of flow cytometry include:

  • Multi-parametric and high-resolution, phenotypic and functional characterization of immune and non-immune cells on a per cell basis.
  • Cellular proliferation
  • Stem, immune, and non-immune cell sorting to high-purity and viability.

Instruments

BD Fortessa X-20

16 color detection on a single cell.

BD FACSAria II

11 color detection on a single cell.
4-way high-purity sorting (>98%).

Scheduling and Calendars

  • Before scheduling time in the Cell Analysis Facility, you must register with the facility, obtain facility training, and have all compliance issues approved prior to performing work.
  • To schedule time on either the FACSAria or the Fortessa, please send email to Robbie Moore, Flow Cytometry Specialist at com-caf@medicine.tamhsc.edu.
  • Please indicate which instrument and whether requesting a “cell sort” or “cell analysis”.
  • Please request appointments at least 2 days in advance.
  • FACS Operator will confirm instrument availability and appointment.
  • CANCELLATIONS & NO-SHOWS: Appointments canceled>24 hours prior to appointment require no charge. Appointments canceled 24-12 hours prior to scheduled time will be charged 1-hour service; appointments canceled less than 12 hours prior to scheduled time will be charged 1.5-hour service; “No-shows” will be charged full scheduled time or 2 hours service, whichever is greater.

Fee Structure

Service MPIM COM-TAMHSC TAMUS Non-TAMUS academic Private
Analysis
Analysis w/ Operator
(Aria & Fortessa)
$40/hr $50/hr
$65/hr
125% 150% 200%
Sort
Sort w/ operator
(Aria only)
$50/hr $70/hr
$85/hr
125% 150% 200%
Microbial Analysis
Microbial Sort
Microbial Sort w/ Operator
(Aria or Fortessa)
$60/hr $85/hr
$95/hr
$105/hr
125% 150% 200%
Training Session
(incl. 2 hrs free pilot exp. Time & initial consultation)
FREE $150 per session 125% 150% 200%
Workstation Analysis FREE FREE 125% 150% 200%
Consultation & Exp Development $50/hr $100/hr 125% 150% 200%
Collaberations Per Case Basis Per Case Basis 125% 150% 200%

Training

  • Before scheduling time in the Cell Analysis Facility, you must register with facility, obtain facility training, and have all compliance issues approved prior to performing work.
  • To schedule time for training on either the FACSAria or the Fortessa, please send email to Robbie Moore Flow Cytometry Specialist at com-caf@medicine.tamhsc.edu.
  • Please indicate which instrument and whether requesting a “new user” or “specific training”.
  • FACS Operator will confirm training availability and appointment date.

External Links

Fluorophore Spectrum Viewers:

Invitrogen:

Fluorescence SpectraViewer

Becton-Dickinson:

BD Fluorescence Spectrum Viewer

Flow Cytometry Tutorials:

Invitrogen:

Becton-Dickinson:

Resources and Vendors: