College of Medicine Cell Analysis Facility

Instruments

BD Fortessa X-20

4 Lasers

• Violet (405)
• Blue (488)
• Yellow-Green (561)
• Red (640)
• 16 fluorescent channels
• Multi-parametric and high-resolution phenotypic and functional characterization on a per cell basis.

BDFACS Aria III

BDFACS Aria III

• Violet (405)
• Blue (488)
• Yellow-Green (561)
• Red (640)
• 13 fluorescent channels
• Up to 4-way bulk sorting
• ACDU attachment for sorting into well plates or slides
• Single Cell Index sorting

Milltenyi gentleMACS Octo Dissociator

Milltenyi gentleMACS Octo Dissociator

• Semi-automated dissociation of tissues into single-cell suspensions or homogenates
• Optimized, pre-set programs
• Kits optimized for specific tissue types: mouse, human or rat-tumor, heart, neural tissue, spleen, lung, lamina propria, muscle, epidermis, liver and others

Seahorse XFe96 Analyzer

Milltenyi gentleMACS Octo Dissociator

• 96-well plate format
• Measure OCR and ECAr of live cells
• Measure mitochondrial function
• Quantify ATP production
• Generate a metabolic phenotype within one hour
• Measure glycolytic rates in live cells
• Flexible assay design

Scheduling and Calendarsa.

All instrument scheduling is done through our iLab website. You must register for an iLab account before scheduling any instruments in the COM-CAF.

To register for an account:

To get started, you must register for an account:

1. Navigate to the core page: https://tamu.corefacilities.org/service_center/show_external/4807
2. In the upper-right-hand corner of the screen where it says, ‘Welcome TAMU user, please click here to login or register,’ select the click here link
3. You will be directed to an authentication page where you will need to enter your TAMU credentials
4. Once you have entered your credentials, click the ‘Login’ button
5. You will be directed to an iLab Registration page where you will need to select your PI/Lab, and verify your contact information
6. Once your registration has been submitted, your PI will receive a notification that you have requested membership to their lab in iLab. They will need to approve your membership and assign any TAMU Fund Name for your use.

To Create an Equipment Reservation:

Once you have been accepted into your PI’s lab and assigned TAMU Fund Name, you can schedule equipment time.

1. Navigate to the core page: https://tamu.corefacilities.org/service_center/show_external/4807
2. At the upper right hand of the page where it says, ‘Welcome TAMU user, click here to log in or register,’ select click here.
3. Enter your TAMU Credential and password, and sign in.
4. Select the Schedule Equipment tab and click on the ‘View Schedule’ button next to the instrument of interest. Click and drag on the time frame you would like to schedule your reservation for.
5. A window will pop up that will allow you to verify your reservations details and provide payment information before saving the reservation.

In addition, before scheduling time in the Cell Analysis Facility, you must obtain facility training. Training is scheduled through iLab. https://tamu.corefacilities.org/sc/4807/college-of-medicine-cell-analysis-facility-com-caf/?tab=about

All compliance issues must be met prior to performing any work in the COM-CAF. If your work in the COM-CAF requires IBC approval, you must add our facility to your protocol as a room amendment. You must also have your agents approved for use on our instruments. We require a copy of your IBC approval letter which states that you have express permission to run your agents on our instruments.

Fee Structure

Training

• Before scheduling time in the Cell Analysis Facility, you must register with facility, obtain facility training, and have all compliance issues approved prior to performing work.
• To schedule time for training on either the FACSAria or the Fortessa, please send email to Robbie Moore Flow Cytometry Specialist at com-caf@medicine.tamhsc.edu.
• Please indicate which instrument and whether requesting a “new user” or “specific training”.
• FACS Operator will confirm training availability and appointment date.

External Links

Fluorophore Spectrum Viewers:

Invitrogen:

Fluorescence SpectraViewer

Becton-Dickinson:

BD Fluorescence Spectrum Viewer

Flow Cytometry Tutorials:

Invitrogen:

• Tutorials

Becton-Dickinson:

• Training
• Tools and Resources

Resources and Vendors:

• Becton-Dickinson
• EBioscience
• BioLegend