Thomas J. Bartosh, Ph.D.

Thomas J. Bartosh, Ph.D.

Assistant Professor, Flow Cytometry Core Supervisor


Phone: 254-771-6845
bartosh@medicine.tamhsc.edu

Education and Post-Graduate Training

TJ Bartosh is an Assistant Professor of Molecular and Cellular Medicine at the Institute for Regenerative Medicine located in Temple, Texas. He received a B.S. degree in Biology from The University of Texas Arlington in 2002. Then, in 2008, he earned his Ph.D. degree in Cell Biology and Genetics from The University of North Texas Health Science Center working with Dr. Rouel S. Roque on the molecular regulation of cardiac stem cell growth and differentiation in 3D cardiac micro-tissue models. Dr. Bartosh joined the Texas A&M University System Health Science Center as a post-doctoral fellow in 2008 working in the laboratory of Dr. Darwin J. Prockop to develop therapies with mesenchymal stem cells from the bone marrow, and in 2011 accepted a position as supervisor of the flow cytometry/cell sorting core laboratory at the Institute for Regenerative Medicine. Dr. Bartosh joined the faculty of Texas A&M Health Science Center in 2012.

Research Interests

There is considerable interest among basic and clinical scientists in exploiting the remarkable therapeutic potential of the stem cells from bone marrow referred to as mesenchymal stem cells or MSCs. The relative ease of MSC procurement and promising results obtained with the cells in models for human disease has encouraged their use in numerous clinical trials. Traditionally, MSCs have been expanded as 2D adherent cultures on plastic surfaces. As stem cells in their native form exist in specialized micro-environments or ‘niches’ the concept that 3D culture approaches could enhance the therapeutic benefits of the cells is of recent interest. The premise behind this hypothesis is that 3D culture recapitulates the spatial organization of the stem cell niche and promotes appropriate intercellular and cell-matrix interactions. These relationships modulate gene expression patterns and can therefore influence cell differentiation and function. Currently, this research laboratory is engaged in the development of 3D culture systems for MSCs to pre-activate the cells and better utilize their inherent therapeutic potential often overshadowed by the limitations of conventional culture methods. The properties of MSCs derived from 3D cultures regarding the ability of the cells to home to injured tissues, reduce inflammation, modulate immune function, suppress tumor growth, and activate tissue-resident stem cells are all under investigation. Moreover, this group aims to determine the cellular and molecular cues that drive the therapeutic effects of MSCs.

Selected Publications

  • Bartosh TJ, Wang Z, Rosalas AA, Dimitrijevich SD, Roque RS. (2008) 3D-model of adult cardiac stem cells promotes cardiac differentiation and resistance to oxidative stress. J Cellular Biochemistry; 105(2):612-623.
  • Bartosh TJ, Ylostalo JH, Mohammadipoor A, Bazhanov N, Coble K, Claypool, K, Lee RH, Choi H, Prockop DJ. (2010) Aggregation of human mesenchymal stromal cells (MSCs) into 3D spheroids enhances their anti-inflammatory properties. Proc Natl Acad Sci U.S.A.; 107(31):13724-9.
  • Roddy GW, Oh JY, Lee RH, Bartosh TJ, Ylostalo J, Coble K, Rosa RH Jr, Prockop DJ. (2011) Action at a distance: systemically administered adult stem/progenitor cells (MSCs) reduce inflammatory damage to the cornea without engraftment and primarily by secretion of TNF-α stimulated gene/protein 6. Stem Cells; 29(10):1572-9.
  • Roddy GW, Rosa Jr RH, Youn Oh J, Ylostalo JH, Bartosh TJ, Choi H, Lee RH, Yasumura D, Ahern K, Nielsen G, Matthes MT, Lavail MM, Prockop DJ. (2012) Stanniocalcin-1 rescued photoreceptor degeneration in two rat models of inherited retinal degeneration. Mol Ther; 20(4):788-97.
  • Ylostalo JH, Bartosh TJ, Coble K, Prockop DJ. (2012) Human mesenchymal stem/stromal cells cultured as spheroids are self-activated to produce prostaglandin E2 that directs stimulated macrophages into an anti-inflammatory phenotype. Stem Cells; 30(10):2283-96.
  • Bartosh TJ, Ylostalo JH, Bazhanov N, Kuhlman J, Prockop DJ. (2013) Dynamic compaction of human mesenchymal stem/precursor cells (MSC) into spheres self-activates caspase-dependent IL1 signaling to enhance secretion of modulators of inflammation and immunity (PGE2, TSG6, and STC1). Stem Cells; 31(11):2443-2456.
  • Ylostalo JH and Bartosh TJ. (2013) Mesenchymal Stem Cells: Characteristics, Niches and Applications for Cell Therapy. Encyclopedia of Molecular Cell Biology and Molecular Medicine, Stem Cells: From Biology to Therapy; Volume 2:429-470.
  • Foskett A, Bazhanov N, XinYu T, Tiblow A, Bartosh TJ, Prockop DJ. (2014) Phase-directed therapy: TSG-6 targeted to early inflammation improves bleomycin-injured lungs. Am J Physiol Lung Cell Mol Physiol; 306(2):L120-31.
  • Bartosh TJ and Ylostalo JH (2014) Preparation of Anti-inflammatory Mesenchymal Stem/Stromal Cells (MSC) Through Spheroid Formation Using Hanging Drop Culture Technique. Current Protocols in Stem Cell Biology; 28:2B.6.1-2B.6.23.
  • Bartosh TJ, Wang Z, Downey HF, Roque RS. (2014) Multipotent Progenitor Cells from the Adult Dog Heart Develop Into Organotypic Cultures of Functional Myocardium. International Journal of Advances in Science and Technology.
  • Bartosh TJ and Ylostalo JH (2014) Model of MSC Aggregate Formation In Vivo. Bio-Protocol.